| 辐射联合p53腺病毒重组体转染对肿瘤细胞的影响 |
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| 引用本文： | 刘兵,张红,高清祥,罗晓红,谢漪,郝冀芳,段昕,周清明,周光明.辐射联合p53腺病毒重组体转染对肿瘤细胞的影响[J].辐射研究与辐射工艺学报,2007,25(3):175-179. |
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| 作者姓名： | 刘兵 张红 高清祥 罗晓红 谢漪 郝冀芳 段昕 周清明 周光明 |
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| 作者单位： | 中国科学院近代物理研究所,兰州,730000;中国科学院研究生院,北京,100039;兰州军区疾病控制中心,兰州,730020;中国科学院近代物理研究所,兰州,730000;兰州大学生命科学院,兰州,730000;兰州军区疾病控制中心,兰州,730020;中国科学院近代物理研究所,兰州,730000;中国科学院研究生院,北京,100039 |
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| 基金项目： | 国家自然科学基金
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甘肃省科技攻关项目
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甘肃省自然科学基金 |
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| 摘 要： | 以绿色荧光蛋白腺病毒重组体(Replication deficient adenovirus green fluorescence protein recombinant,AdCMV-GFP)为对照,用p53腺病毒重组体(Replication deficient adenovirus p53 recombinant,AdCMV-p53)转染经0、0.25、0.5、1.0、1.5和2.0Gy γ射线预辐射的HepG2(wtp53)、Hela(wtp53,wtP53低水平表达)和HT-29(mtp53,mtP53过表达)细胞,用克隆形成法检测肿瘤细胞存活,探讨AdCMV-p53转染对p53基因状态与功能不同肿瘤细胞辐射敏感性的影响.结果显示,AdCMV-p53转染不仅明显提高肿瘤细胞辐射敏感性,而且与肿瘤细胞内在p53基因状态与功能有关.
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| 关 键 词： | p53基因 辐射 腺病毒载体 肿瘤细胞 辐射敏感性 |
| 修稿时间： | 2007-01-212007-03-13 |
Effect of combination of AdCMV-p53 and irradiation on tumor cells |
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| LIU Bing,ZHANG Hong,GAO Qingxiang,LUO Xiaohong,XIE Yi,HAO Jifang,DUAN Xin,ZHOU Qingming,ZHOU Guangming.Effect of combination of AdCMV-p53 and irradiation on tumor cells[J].Journal of Radiation Research and Radiation Processing,2007,25(3):175-179. |
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| Authors: | LIU Bing ZHANG Hong GAO Qingxiang LUO Xiaohong XIE Yi HAO Jifang DUAN Xin ZHOU Qingming ZHOU Guangming |
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| Affiliation: | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000;Graduate University, Chinese Academy of Sciences, Beijing 100039;Lanzhou Command Center for Disease Prevention and Control, Lanzhou 730020;School of Life Science, Lanzhou University, Lanzhou 730000 |
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| Abstract: | The work is to investigate effect of AdCMV-p53 gene transfer on the radiosensitivity of tumor cells. HepG2 (wtp53), Hela (wtp53, low level wtp53 expression) and HT-29 (mtp53, mtp53 over-expression) cells exposed to 0, 0.25, 0.5, 1.0, 1.5 and 2.0Gy were infected with AdCMV-GFP, a replication deficient recombinant adenoviral vector containing a CMV promoter and green fluorescent protein, or AdCMV-p53, a replication deficient recombinant ade-noviral vector containing a CMV promoter and carrying human wild-type p53 gene. Survival fraction of the cells was determined by clonogenic assay. Survival curves were generated by linear quadratic model. The results showed that AdCMV-p53 infection could significantly promote the radiosensitivity of tumor cells. There is a relationship between the effect of AdCMV-p53 infection and the cellular intrinsic p53 status. |
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| Keywords: | p53 Irradiation Adenovirus vector Tumor cell Radiosensitivity |
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