A biosensor in the time domain based on the diffusion coefficient measurement: intermolecular interaction of an intermediate of photoactive yellow protein |
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Authors: | Khan Javaid Shahbaz Imamoto Yasushi Yamazaki Yoichi Kataoka Mikio Tokunaga Fumio Terazima Masahide |
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Affiliation: | Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan. |
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Abstract: | A new type of biosensor is presented for the first time. This method is based on the diffusion measurement in the time domain by the transient grating method. As the first demonstration of this new method, various intermolecular interactions with a reaction intermediate of photoactive yellow protein, such as the protein-protein interaction, protein-DNA interaction, and protein-small molecule binding are detected. The characteristic advantages and limitations are summarized. |
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