| Abstract: | The extent to which protein synthesis is involved in working memory was investigated with the protein synthesis inhibitor anisomycin (ANI). Male albino and Long-Evans rats were trained to perform accurately on a 12-arm radial maze when delays of 240 min were interposed between Choice 6 and Choice 7. Bilateral hippocampal cannulas were then implanted. Accuracy on Choices 7–22 was studied when ANI (80 μg/μl) or saline was injected either 30 min before Choice 1 or 5–20 min after Choice 6 in Exp I. Pretrial injection of ANI significantly impaired performance following the 240-min delay, whereas ANI injected during the delay had no such effect. In Exps II and III, the ANI-induced amnesia was replicated, and the temporal course of development of the amnesia was determined. Pretrial administration of ANI did not significantly affect retention after a 2-min delay but produced amnesia after delays of 15 min or longer. Data suggest that protein synthesis is important for the formation of temporary memories, provided the retention interval is long enough. It is suggested that working memory includes both short- and long-term components. Protein synthesis appears to be important for formation of the long-term component, but not the short-term component, of working memory. (31 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) |
