特异性抗幽门螺杆菌IgY的制备和活性测定

目的寻找一种高效的特异性抗幽门螺杆菌IgY的制备和活性测定方法。方法分别用福氏完全佐剂、福氏不完全佐剂混合含幽门螺杆菌灭活全菌及其超声碎片的试剂,对6只31周海兰褐种产蛋母鸡进行免疫,2周后二免,4周后三免。于初免后即开始收集鸡蛋,提取特异性抗幽门螺杆菌IgY,采用间接血凝试验测定卵黄中的抗体效价,体外抑菌试验测定其抑制幽门螺杆菌的活性。结果母鸡在初免2周后,抗体效价可达1∶256,初免3周后,抗体效价可达1∶1 024,初免4周后,抗体效价可达1∶8 192;同批样品在此水平可维持达11周以上,然后抗体效价呈缓慢下降趋势,26周后抗体效价仍可保持在较高水平。特异性抗幽门螺杆菌IgY具有明显的抑制HP生长的活性。结论采取加强免疫方法,可高效地引起免疫应答,是一种较好的获得抗体的方法。

Preparation and Evaluation of Specific Yolk Antibodies Against H.Pylori

Objective To find an effective way to prepare the specific Yolk antibodies against Helicobacter pylori（HP） and to evaluate the antibody titer.Methods Six 31-week-old Hy-Line laying hens were immunized with inactivated HP antigen and its ultrasonic HP fragment antigen combined with either Freund＇s complete adjuvant or Freund＇s incomplete adjuvant.The second immunization was performed after 2 weeks,and the third immunization was taken after 4 weeks.The eggs were collected after the first immunization and the specific Yolk antibodies anti-HP were prepared.The immune response was detected by IHA and the bioactivity and titer of anti-HP IgY were evaluated.Results Two weeks after the first immunization,the titer of the antibodies reached 1∶256.Three weeks after the first immunization,the titer reached 1∶1 024.Four weeks after the first immunization,the titer reached 1∶8 192 and was maintained at this level for more than 11 weeks,then the titer dropped slightly.After 26 weeks,the titer still was maintained at a high level.In addition,IgY-HP restrained the growth of HP.Conclusion Booster immunization is a good way to acquire the effective antibodies.