| 盐酸氨基葡萄糖反相高效液相色谱法含量测定方法研究 |
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| 引用本文： | 万慧芳,万欢,汪华杰.盐酸氨基葡萄糖反相高效液相色谱法含量测定方法研究[J].矿产勘查,2010(5):96-97,99. |
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| 作者姓名： | 万慧芳 万欢 汪华杰 |
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| 作者单位： | [1]南昌大学医学院医学实验教学部,南昌330006 [2]江西省医药学校教务科,南昌330200 |
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| 摘 要： | 目的建立盐酸氨基葡萄糖反相高效液相色谱法（RP-HPLC）的含量测定方法。方法采用RP-HPLC。色谱柱：C18（4.6 mm×100 mm,5μm）,流动相：三水合乙酸钠溶液-甲醇（80：20）,检测波长340 nm,柱温20℃,流速1.0 mL·min^-1。结果盐酸氨基葡萄糖在0.1～1.0 g·L^-1范围内呈良好的线性关系（r^2=0.999 8）,平均回收率为96.79%,RSD为0.89%。结论本研究建立的盐酸氨基葡萄糖RP-HPLC含量测定方法准确、简便、专属性强。
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| 关 键 词： | 盐酸氨基葡萄糖 反相高效液相色谱法 含量测定 |
Determination of Glucosamine Hydrochloride by RP-HPLC |
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| WAN Hui-fang,WAN Huan,WANG Hua-jie.Determination of Glucosamine Hydrochloride by RP-HPLC[J].Mineral Exploration,2010(5):96-97,99. |
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| Authors: | WAN Hui-fang WAN Huan WANG Hua-jie |
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| Affiliation: | 1.Department of Medical Experiment Teaching,Medicine College of Nanchang University,Nanchang 330006,China;2.Academic Division,Jiangxi Medical School,Nanchang 330200,China) |
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| Abstract: | Objective To establish a RP-HPLC method for determination glucosamine hydrochloride.Methods By RP-HPLC,C18 column（4.6 mm×100 mm,5 μm）was used and the mobile phase was a mixture of sodium acetate trihydrate and methanol（80∶20）.The flow rate was 1.0 mL·min^-1 and the detective wavelength was 340 nm,and the column temperature was held at 20 ℃.Results Within the range of 0.1～1.0 g·L^-1,there was a good linear relation ship between the concentration and the peak area of glucosamine hydrochloride（r=0.999 8）.The average recovery was 96.79%（RSD was0.89%）.Conclusions RP-HPLC is accurate,simple and specific and can be applied to determine the content of glucosamine hydrochloride. |
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| Keywords: | glucosarnine hydrochloride RP-HPLC determination |
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