| 胶质瘤组织中人类巨细胞病毒的感染研究 |
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| 引用本文： | 曾而明,罗达亚,王红梅,李东海,洪涛. 胶质瘤组织中人类巨细胞病毒的感染研究[J]. 矿产勘查, 2010, 0(6): 1-3 |
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| 作者姓名： | 曾而明 罗达亚 王红梅 李东海 洪涛 |
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| 作者单位： | [1]南昌大学第一附属医院神经外科,南昌330006 [2]南昌大学基础医学院生物化学与分子生物学教研室,南昌330006 [3]南昌大学基础医学院病理生理学教研室,南昌330006 |
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| 基金项目： | 江西省教育厅科技计划（赣教技字[2007]92号）; 江西省卫生厅科技计划（20063081）,致谢：感谢安徽医科大学微生物教研室王明利教授惠赠人类巨细胞病毒以利于研究. |
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| 摘 要： | 目的探讨人类巨细胞病毒（HCMV）在胶质瘤组织中的表达,为阐述HCMV感染在胶质瘤发病中的作用提供依据。方法以HCMV糖蛋白B（UL55）基因设计引物,对62例不同级别星形细胞瘤、少突胶质细胞瘤组织和7例外伤脑组织DNA进行巢式PCR检测。结果 62例中58例检测出HCMV基因片段,7例正常脑组织未检测出HCMV基因片段。结论星形细胞瘤、少突胶质细胞瘤患者存在HCMV感染,HCMV感染可能在胶质瘤发生、发展中起作用。
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| 关 键 词： | 胶质瘤 人类巨细胞病毒 致病因素 HCMV基因组 |
Expression of Human Cytomegalovirus Infection in Gliomas Tissue |
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| ZENG Er-ming,LUO Da-ya,WANG Hong-mei,LI Dong-hai,HONG Tao. Expression of Human Cytomegalovirus Infection in Gliomas Tissue[J]. Mineral Exploration, 2010, 0(6): 1-3 |
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| Authors: | ZENG Er-ming LUO Da-ya WANG Hong-mei LI Dong-hai HONG Tao |
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| Affiliation: | 1.Department of Neurosurgery,the First Affiliated Hospital;2.Department ofBiochemistry and Molecular Biology,Basic Medical College;3.Department of Pathophysiology,Basic Medical College,Nanchang University,Nanchang 330006,China) |
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| Abstract: | Objective To investigate the gene expression of human cytomegalovirus（HCMV） in glioma tissue and to explore the relationship between HCMV infection and gliomas.Methods Glycoprotein B（gB） gene was used as a target for detection of HCMV to design two pairs of primers.HCMV genome DNA in 62 cases of gliomas tissues of astrocytic tumors and oligodendroglial tumours and 7 normal brain tissues was detected by nested PCR.Results HCMV genome DNA expressed in 58 gliomas tissues,but was not be found in normal brain tissues.Conclusion HCMV infection is present in glioma tissues and may play a potential role in the development of glioma. |
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| Keywords: | glioma cytomegalovirus pathogens carcinogenesis human cytomegalovirus gene group |
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