(1) Department of Plant Science, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada;(2) Grain Research Laboratory, Canadian Grain Commission, 1014-303 Main Street, R3C 3G8 Winnipeg, Manitoba, Canada
Abstract:
Lipids of canola seedcoats (Brassica napus L. andB. rapa L.) were prepared by surface washing and by complete extraction of seed coats with toluene. The major fatty acyl-containing
triacylglycerols, wax esters and free fatty acids were separated by thin-layer chromatography prior to transesterification
and analysis by gas-liquid chromatography. The proportion of C18∶1n−7 to C18∶1n−9 was higher in the extracted lipids than
in the surface-washed lipids for all three classes.