VH gene family utilization of anti-acetylcholine receptor antibodies in experimental autoimmune myasthenia gravis |
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Authors: | YM Graus JJ Verschuuren NA Bos PJ van Breda Vriesman MH De Baets |
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Affiliation: | Department of Immunology, University of Limburg, Maastricht, Netherlands. |
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Abstract: | The immunoglobulin heavy chain (VH) gene family usage in experimental autoimmune myasthenia gravis (EAMG) model was investigated by RNA slot blot hybridization using VH gene family specific probes. Anti-acetylcholine receptor (AChR) monoclonal antibodies (mAbs) isolated from susceptible C57BL/6 and resistant BALB/c mice were found to be encoded by VH genes from at least six different families. The Vgam3.8 family was overrepresented in alpha-bungarotoxin blocking mAbs. Expression of cross-reactive idiotypes by anti-AChR mAbs was irrespective of the VH gene family usage. Strain dependent differences in susceptibility for EAMG were not reflected in an aberrant VH gene family usage of anti-AChR mAbs. |
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