| 响应面法优化酶法水解大豆异黄酮的条件 |
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| 引用本文： | 岑俊静,汪钊.响应面法优化酶法水解大豆异黄酮的条件[J].食品研究与开发,2009,30(7). |
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| 作者姓名： | 岑俊静 汪钊 |
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| 作者单位： | 浙江工业大学,生物与环境工程学院,浙江,杭州,310014 |
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| 摘 要： | 在单因素试验基础上,利用响应面分析(response surface analysis,RSA)法中Plackett-Burman和Box-Behnken进行设计,得出β-葡萄糖苷酶水解大豆异黄酮物质的最佳条件为:水解时间55 min,水解温度57℃,pH3.5.在最佳条件下,用β-葡萄糖苷酶水解大豆异黄酮得到大豆苷元,其得率达到39.05%.
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| 关 键 词： | 响应面分析法 β-葡萄糖苷酶 水解 大豆异黄酮 β-glucosidase |
OPTIMAL HYDROLYSIS OF ISOFLAVONE WITH ENZYME BY RSA |
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| CEN Jun-jing,WANG Zhao.OPTIMAL HYDROLYSIS OF ISOFLAVONE WITH ENZYME BY RSA[J].Food Research and Developent,2009,30(7). |
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| Authors: | CEN Jun-jing WANG Zhao |
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| Abstract: | On the base of the single factor experiments,designed with the Plackett-Burman and Box-Behnken in the RSA software,an optimum condition the hydrolysis of soybean isoflavone byβ-glucosidase were:55 min of hydrolysis, 57℃ of the temperature,pH3.5. The conversion of daizein is 39.05 %. |
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| Keywords: | RSA hydrolyze soybean isoflavone |
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