Quantitative content of total polar lipids in soybean seeds

The exhaustive stepwise extraction of soybean seeds was performed in a specially designed apparatus by a solvent system containing chloroform, methanol, water, mineral acid, and antioxidant in an inert atmosphere. The yield of total lipids was as high as 99.95%. The possible artifact content in the extracted lipids was < 0.5%. Separation of polar lipids and determination of constituent groups of these compounds (fatty acyl groups and phosphate) has shown that 36.7 μmole (±4.6%) of polar lipids may be contained in 1 g dry wt of seeds, 25.9 μmole (±1%) of this amount being phospholipids.