基于像面数字全息术的中药饮片细胞定量成像技术研究

细胞三维定量成像为中药饮片显微鉴别提供了新方法。为了提高数字全息显微成像质量,采用理论分析与实验验证相结合的方法,对球面参考光像面数字全息显微术的记录和再现过程进行了研究,提出了利用标准分辨率测试板对系统放大倍数、物距等参数进行标定的方法;并利用实验结果对两种常见的相位解包裹方法进行了对比。结果表明:球面参考光像面数字全息图不仅具有较高的信息容量,而且再现过程非常简单,还可以在记录过程中实时观察被记录样品的情况,并选择恰当的被记录区域。利用美国空军分辨率测试板的强度再现像就可以对全息成像系统的放大倍数等参数进行精确标定;利用基于横向剪切的最小二乘解包裹方法可以得到具有较大纵深细胞的准确相位;采取边缘识别技术,可以提高细胞再现像显示效果。

Image-Plane Digital Holography for Quantitative Imaging of Cells of Chinese Medical Decoction Pieces

Quantitative three-dimensional imaging of cells of Chinese medical decoction pieces can provide a new method for microscopic identification of decoction pieces. To improve the digital holographic image quality, the recording and reconstruction processes of image-plane digital micro-hologram recorded with spherical reference light are studied theoretically and experimentally. A method for measuring the recording parameters of the holographic imaging system such as the magnification, object distance by using a standard test target is proposed. The two popular phase-unwrapping methods are compared experimentally. The results show that the image-plane digital micro-holography recorded with spherical reference light is characterized by more information recorded, very easy reconstruction and the real-time observation of the investigated specimen for determining the region correctly. The parameters of the holographic system can be determined accurately by using the reconstructed intensity image of a US Air Force test target. The unwrapped phase with big depth sample can be obtained accurately by using the lateral-shearing-based least square (LSBLS) algorithm. Using the edge reorganization technique the cell image can be better displayed.