Determination of the structure of lecithins via the formation of acetylated 1,2-diglycerides |
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Authors: | O S Privett L J Nutter |
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Affiliation: | (1) The Hormel Institute, University of Minnesota, Austin, Minnesota |
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Abstract: | A detailed procedure for quantitative determinations of molecular species of lecithins is described and applied to several
lecithins isolated from natural sources. The method is based on the conversion of lecithin to acetylated 1,2-diglycerides
and analysis of these compounds by methodology used for the determination of triglyceride structure.
The preparation of the acetylated 1,2-diglycerides was carried out via hydrolysis with phospholipase C and acetylation of
the resultant, 1,2-diglycerides with pyridine-acetic anhydride. Preparation of acetylated 1,2-diglycerides from lecithin by
acetolysis with acetic acid-acetic anhydride was shown to be accompanied by intermolecular as well as intramolecular rearrangement
of the fatty acids.
The structure of the acetylated 1,2-diglycerides was determined by a combination of argentation-TLC and pancreatic lipase
hydrolysis using internal standards for quantification. The method was applied to lecithins isolated from milk serum, egg,
soybean, safflower seed and wheat germ lipids. |
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