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| 阳离子交换型连续床用超大孔晶胶的制备 | |
| 引用本文： | 王良华,陈芳,贠军贤,姚克俭,姚善泾.阳离子交换型连续床用超大孔晶胶的制备[J].高校化学工程学报,2009,23(3). |
| 作者姓名： | 王良华 陈芳 贠军贤 姚克俭 姚善泾 |
| 作者单位： | 1. 浙江大学,化学工程与生物工程学系,浙江,杭州,310027;浙江工业大学,化学工程与材料学院,绿色化学合成国家重点实验室培育基地,浙江,杭州,310032 2. 浙江工业大学,化学工程与材料学院,绿色化学合成国家重点实验室培育基地,浙江,杭州,310032 3. 浙江大学,化学工程与生物工程学系,浙江,杭州,310027 |
| 摘 要： | 采用原位接枝法,将3-烯丙氧基-2-羟基-1-丙磺酸钠(AHPSA)接枝到聚丙烯酰胺基晶胶基质孔隙内表面,制备得到了阳离子交换型连续床用的带磺酸基超大孔晶胶介质.通过脉冲示踪法测量停留时间分布(RTD),得到了晶胶介质的理论等板高度(HETP).通过测量一定压差下流经晶胶床柱的液量,得到晶胶介质的渗透率.用溶菌酶作为模型蛋白测量晶胶介质的吸附容量.考察了单体浓度、接枝反应时间等反应条件对晶胶介质的HETP、渗透率、蛋白质吸附容量等性能的影响.结果表明:接枝AHPSA的阳离子交换晶胶介质的HETP基本不受接枝反应时间和单体浓度的影响,其渗透率随接枝反应时间增大略有减小,其对溶菌酶的吸附容量与接枝单体的浓度成正比,受接枝反应时间的影响较小. |
| 关 键 词： | 生物分离 超大孔连续床 原位接枝 晶胶 阳离子交换 |
Preparation of Supermacroporous Cryogel Used in Cation-Exchange Monolith |
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| WANG Liang-hua,CHEN Fang,YUN Jun-xian,YAO Ke-jian,YAO Shan-jing.Preparation of Supermacroporous Cryogel Used in Cation-Exchange Monolith[J].Journal of Chemical Engineering of Chinese Universities,2009,23(3). | |
| Authors: | WANG Liang-hua CHEN Fang YUN Jun-xian YAO Ke-jian YAO Shan-jing |
| Affiliation: | 1.Department of Chemical and Biochemical Engineering;Zhejiang University;Hangzhou 310027;China;2.State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology;College of Chemical Engineering and Materials Science;Zhejiang University of Technology;Hangzhou 310032;China |
| Abstract: | A novel supermacroporous cryogel with sulfo binding groups used in cation-exchange monolith was prepared by in-situ grafting of 3-allyloxy-2-hydroxy-1-propanesulfonic acid sodium salt (AHPSA) onto the pore surfaces of polyacrylamide-based cryogel. The height equivalent to a theoretical plate (HETP) of the cryogel beds was obtained by measuring residence time distributions (RTDs) with tracer pulse-response method. The hydraulic permeability was calculated by measuring the flow rate of deionized water passing... |
| Keywords: | bioseparation supermacroporous monolith in-situ graft-polymerization cryogel cation-exchange |
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