| 从啤酒酵母中提取RNA新工艺的研究 |
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| 引用本文： | 张帅. 从啤酒酵母中提取RNA新工艺的研究[J]. 中国酿造, 2005, 0(2): 17-19 |
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| 作者姓名： | 张帅 |
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| 作者单位： | 广东省肇庆学院,广东,肇庆,526061 |
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| 摘 要： | 研究了从啤酒酵母中提取RNA的新生产工艺,即在均质法和氨解法的基础上,将两者结合为均质- 氨解法。得出最佳工艺条件:酵母浓度10%,均质破壁循环2次,氨浓度0.6%,三氯乙酸浓度4%,提取30min。将上清液循环回用,RNA平均净得率>3%,第3次循环时净得率最高。
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| 关 键 词： | 均质 啤酒酵母 得率 生产工艺 新工艺 酸浓度 三氯乙酸 氨解法 最佳工艺条件 研究 |
| 文章编号： | 0254-5071(2005)02-0017-03 |
| 修稿时间： | 2004-07-21 |
Study on new technique of RNA extraction from Saccharomyces cerevisiae |
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| ZHANG Shuai. Study on new technique of RNA extraction from Saccharomyces cerevisiae[J]. China Brewing, 2005, 0(2): 17-19 |
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| Authors: | ZHANG Shuai |
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| Abstract: | s: A new process technology of RNA extraction from Saccharomyces cerevisiae was studied, based on the combination of homogenization and ammonification. The best technique condition was as follows, (1) the concentration of Saccharomyces cerevisiae, ammonia and trichloroacetic acid was 10%, 0.6% and 4%, respectively; (2) cell wall breaking by homogenization two times; (3) the time of RNA extraction was 30 min. The average yield of RNA was more than 3% and the yield was the highest when the supernatant was recycled at the third time. |
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| Keywords: | Saccharomyces cerevisiae RNA homogenization ammonia |
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