纤维藻培养及制备生物柴油的研究

探讨了纤维藻(Ankistudesmus sp)的低成本培养模式,考察了氮源和碳源以及反应器形式对纤维藻生物量、油脂积累以及油脂组成的影响。户外培养纤维藻在氮饥饿条件下油脂产率较高;通过槽式反应器和管式反应器的比较发现:槽式反应器更适合微藻的大规模培养;混养培养时能显著增加纤维藻的生物量和油脂含量,最佳添加条件下藻的生物量和油脂含量分别高达1.64 g/L和15.9%,1.41 g/L和11.9%。藻油经酸催化甲酯化制备生物柴油,经气相色谱分析,藻油主要成分为棕榈酸、油酸和亚油酸。氮缺陷、流加葡萄糖培养得到的纤维藻油含有25.32%的棕榈酸、44.74%的油酸,其制备得到的生物柴油具有更好的氧化稳定性和低温流动性。

Study on the cultivation of Ankistudesmus sp and preparation for biodiesel production

The low cost cultivation mode of Ankistudesmus sp was discussed in this study.The effect of nitrogen,carbon and reactor pattern on biomass,fat accumulation and fat composition were investigated.Lipid production got higher when in nitrogen starvation cultured outside..Through the comparison between slot reactor and tubular reactor.It discoverd that slot reactor is more suitable for large scale microalgae cultivation.Mixotrophic cultivation could significantly improve algal biomass and lipid content,under the optimal addition conditions,biomass and lipid content were up to 1.41 g/L and 11.9%,1.64 g/L and 15.9% respectively.Algal lipid were made into biodiesel through acidic catalysis and methyl esterification.The main contents of algal lipid were palmitic acid,oleic acid and linoleic acid.Furthermore,the obtained algal oil in conditions of nitrogen limitation and adding glucose had 25.32% palmitic acid and 44.74% oleic acid which could provide better oxidative stability and cold flow properties.