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CE-MS-based proteomics in biomarker discovery and clinical application |
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| Authors: | Claudia Pontillo Szymon Filip Daniel M. Borràs William Mullen Antonia Vlahou Harald Mischak |
| Affiliation: | 1. Department of R&D, Mosaiques Diagnostics GmbH, Hanover, Germany Charité–Universitätsmedizin Berlin, Berlin, Germany These authors contributed equally to this work.;2. Charité–Universitätsmedizin Berlin, Berlin, Germany Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece These authors contributed equally to this work.;3. Department of R&D, ServiceXS, Leiden, The Netherlands Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Cardiovascular and Metabolic Disease, Toulouse, France Université Toulouse III Paul-Sabatier, Toulouse, France These authors contributed equally to this work.;4. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK;5. Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece School of Biomedical and Healthcare Sciences, Plymouth University, Plymouth, UK;6. Department of R&D, Mosaiques Diagnostics GmbH, Hanover, Germany Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK Correspondence: Dr. Harald Mischak, Mosaiques Diagnostics GmbH, Mellendorfer, Strasse 7–9, D-30625 Hannover, Germany E-mail: mischak@mosaiques-diagnostics.com Fax: +49-511-55-47-44-31 |
| Abstract: | CE-MS is applied in clinical proteomics for both the identification of biomarkers of disease and assessment of biomarkers in clinical diagnosis. The analysis is reproducible, fast, and requires only small sample volumes. However, successful CE-MS analysis depends on several critical steps that can be consolidated as follows: (i) proper sample preparation and fractionation, (ii) application of suitable capillary coating and appropriate CE-MS interfaces, to ensure the reproducibility and stability of the analysis, and (iii) an optimized clinical and statistical study design to increase the chances for obtaining clinically relevant results. In this review, we cover all these aspects, and present several examples of the application of CE-MS in clinical proteomics. |
| Keywords: | Biomarker Capillary electrophoresis Clinical proteomics Mass spectrometry Peptidomics |