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State-of-the-art nanoplatform-integrated MALDI-MS impacting resolutions in urinary proteomics |
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| Authors: | Judy Gopal Manikandan Muthu Se-Chul Chun Hui-Fen Wu |
| Affiliation: | 1. Department of Molecular Biotechnology, Konkuk University, Seoul, Korea;2. Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung, Taiwan Center for Nanosciences and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, Taiwan Correspondence: Hui-Fen Wu, Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan E-mail: hwu@faculty.nsysu.edu.twFax: +886-7-5253908 |
| Abstract: | Urine proteomics has become a subject of interest, since it has led to a number of breakthroughs in disease diagnostics. Urine contains information not only from the kidney and the urinary tract but also from other organs, thus urinary proteome analysis allows for identification of biomarkers for both urogenital and systemic diseases. The following review gives a brief overview of the analytical techniques that have been in practice for urinary proteomics. MALDI-MS technique and its current application status in this area of clinical research have been discussed. The review comments on the challenges facing the conventional MALDI-MS technique and the upgradation of this technique with the introduction of nanotechnology. This review projects nano-based techniques such as nano-MALDI-MS, surface-assisted laser desorption/ionization, and nanostructure-initiator MS as the platforms that have the potential in trafficking MALDI-MS from the lab to the bedside. |
| Keywords: | Biomarkers Detection MALDI-MS Nanoparticle assisted Urinary proteomics |