| 小分子封闭提高固定化青霉素酰化酶的稳定性 |
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| 引用本文： | 周成,王安明,祝社民,沈树宝.小分子封闭提高固定化青霉素酰化酶的稳定性[J].化学工程,2010,38(2). |
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| 作者姓名： | 周成 王安明 祝社民 沈树宝 |
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| 作者单位： | 1. 南京工业大学,生物与制药工程学院,材料化学工程国家重点实验室,江苏,南京,210009
2. 南京工业大学,材料科学与工程学院,材料化学工程国家重点实验室,江苏,南京,210009 |
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| 基金项目： | 十一五国家863项目(2006AA02Z211);;国家自然科学基金资助项目(20376034);;江苏省自然科学基金资助项目(BK2006181);;江苏省高校研究生创新计划项目(2007) |
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| 摘 要： | 为提高青霉素酰化酶的催化性能和热稳定性,在酶组装过程中添加小分子试剂对介孔泡沫硅载体表面过量的活化位点进行封闭。详细考察了小分子添加质量分数和种类对青霉素酰化酶负载率、催化活力及热稳定性的影响。实验结果得到:经精氨酸封闭的固定化酶活力提高至1.92倍;甘氨酸封闭的固定化酶5 h的50℃热稳定性提高至2.9倍,甘氨酸和谷氨酸封闭的固定化酶50℃热处理25 h仍保持87.9%和82.2%的残余活力;甘氨酸和谷氨酸封闭的固定化酶最适催化pH值向中性偏移且对pH值的耐受性增强。结果表明,在青霉素酰化酶共价组装过程中添加合适的小分子封闭能显著提高酶的催化性能和热稳定性。
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| 关 键 词： | 青霉素酰化酶 固定化酶 介孔泡沫硅 精氨酸 甘氨酸 封闭 |
Improvement for catalytic stability of immobilized penicillin acylase by blockage of small molecule reagents |
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| ZHOU Cheng,WANG An-ming,ZHU She-min,SHEN Shu-bao.Improvement for catalytic stability of immobilized penicillin acylase by blockage of small molecule reagents[J].Chemical Engineering,2010,38(2). |
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| Authors: | ZHOU Cheng WANG An-ming ZHU She-min SHEN Shu-bao |
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| Affiliation: | 1.State Key Laboratory of Materials-Oriented Chemical Engineering;College of Life Science and Pharmaceutical Engineering;2.State Key Laboratory of Materials-Oriented Chemical Engineering;College of Materials Science and Engineering;Nanjing University of Technology;Nanjing 210009;Jiangsu Province;China |
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| Abstract: | In order to improve catalytic properties and thermal stability of penicillin acylase(PA),small molecule reagents were assembled to quench the excessive active groups of mesocellular siliceous foams(MCFs).The effects of adding mass fraction and types of small molecule reagents on the coupled yield,the catalytic activity and thermal stability of PA were investigated.The activity of immobilized PA quenched by arginine is enhanced to 1.92 folds,and the thermal stability of immobilized PA preparation blocked by ... |
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| Keywords: | penicillin acylase immobilized enzyme mesocellular siliceous foams arginine glycin blockage |
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