| 产纤维素酶霉菌的筛选及初步鉴定 |
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| 引用本文： | 陈燕,王小芬,金伟,胡申才,曾驰.产纤维素酶霉菌的筛选及初步鉴定[J].武汉工业学院学报,2011(3):6-8,13. |
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| 作者姓名： | 陈燕 王小芬 金伟 胡申才 曾驰 |
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| 作者单位： | 武汉工业学院生物与制药工程学院; |
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| 基金项目： | 湖北省教育厅科学项目(B20111701); 武汉工业学院校立科研项目(2009Q08);武汉工业学院校级大学生创新性实验计划项目(CX200909) |
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| 摘 要： | 在长期有腐烂植物的潮湿处采集8种样品,用羧甲基纤维素液体培养基富集培养,分离得到34株产纤维素酶的霉菌。利用刚果红染色法对所得菌株进行染色并以水解圈/菌落直径大小为筛选标准进行初筛,得到11株菌。再用DNS法测定初筛所得菌株发酵液中纤维素酶的活力,得到两株纤维素酶活性较高的菌株wx0503、wx1005,其发酵液纤维素酶活力分别可达31.31 U/mg、47.46 U/mg。此外,用苏丹混合液染色法对初筛所得到的产纤维素酶菌株染色,镜检观察其产油脂情况,得到2株产油量较大的纤维素酶产生菌株wx0403、wx1007。
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| 关 键 词： | 纤维素酶 真菌 酶活 油脂 |
Screening and characterization of cellulase-producing fungi strains |
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| CHEN Yan,WANG Xiao-fen,JIN Wei,HU Shen-cai,ZENG Chi.Screening and characterization of cellulase-producing fungi strains[J].Journal of Wuhan Polytechnic University,2011(3):6-8,13. |
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| Authors: | CHEN Yan WANG Xiao-fen JIN Wei HU Shen-cai ZENG Chi |
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| Affiliation: | CHEN Yan,WANG Xiao-fen,JIN Wei,HU Shen-cai,ZENG Chi(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China) |
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| Abstract: | In this study,eight samples were collected from moist environments with plants decaying,in which thirty-four cellulase-producing fungi strains were isolated through enrichment culturing by CMC-Na liquid medium.These cellulase-producing fungi strains were then screened using congo red staining and eleven strains producing big hydrolysis circle were selected.Subsequently,the DNS method was employed to screen the selected strains.Two strains,wx0503 and wx1005,were shown to produce the highest cellulase activit... |
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| Keywords: | cellulase fungi enzyme activity oil |
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