多种生姜有效成分联合提取工艺

为了研究生姜中有效成分姜辣素、姜黄素以及生姜蛋白酶同步提取的方法,文中使用乙醇溶剂法结合旋蒸确定了生姜中3种有效成分同步提取的工艺条件,即生姜与无水乙醇料液比1∶3(g∶mL),生姜蛋白酶提取中缓冲液pH值为6.5,冰浴时间7 h;姜辣素、姜黄素旋转蒸发最佳温度为60℃,转速为16 r/min。在此优化条件下,生姜中姜辣素、姜黄素的提取率分别为0.3143%、0.3889%;生姜蛋白酶的得率为1.376%,比活力达到4 465 U/mg。此工艺能够同步提取姜辣素、姜黄素以及生姜蛋白酶3种有效成分,减少了原材料的消耗,降低生产成本,更适合产业化生产。

The optimum extraction process of ginger various effective components

In order to study the effective extraction of ginger oleoresin,ginger protease and curcumin simultaneous extraction,ethanol solvent combined with rotary evaporation was studied.The ratio of ginger and ethanol liquid ratio was 1∶ 3 g∶ mL),extraction buffer pH 6.5,ice bath time 7h;for the ginger oleoresin and curcumin rotary evaporation,the optimum temperature was 60℃,the rotating speed was 16 r/min.Under the above optimized conditions,gingerol in ginger,curcumin extraction rate was 0.314 3% and 0.388 9%;ginger protease ratio was 1.376%,specific enzymatic activity reached to 4 465 U/mg.This process can simultaneously extract gingerol,curcumin and ginger protease three effective components,reduce the consumption of raw materials,the production cost,and therefore more suitable for industrial production.