超临界CO_2萃取柚子叶、花精油的GC-MS分析

采用超临界CO2萃取技术提取柚子叶、花中的精油,运用气相色谱-质谱(GC-MS)联用技术对其化学成分进行分离和鉴定,并用峰面积归一化法确定各组分的相对含量。结果表明:从柚子叶精油中分离出61个色谱峰,鉴定出39个化合物,占总离子峰相对含量的90.05%。其主要化学成分有匙叶桉油烯醇(21.36%),石竹烯氧化物(17.16%),β-石竹烯(8.40%),α-石竹烯(2.07%),异芳萜烯氧化物(2.01%)等。从柚子花精油中分离出42个色谱峰,鉴定出27个化合物,占总离子峰相对含量的89.31%。其主要化学成分有金合欢醇(19.02%)、橙花叔醇(18.24%),龙脑(6.60%),芳樟醇(4.03%),橙花醇(2.57%)等。柚子叶与柚子花精油共同鉴定出的化合物有橙花醇、香叶醇、香叶酸、β-石竹烯等12种,其相对含量存在不同程度差异。

GC-MS analysis of essential oil by supercritical CO2fluid extraction from pomelo leaf and flower

Essential oil of pomelo leaf and flower was extracted by supercritical CO2 fluid extraction technology.Their compositions and relative contents were quantitatively determined by GC-MS with peak area normalization method.Results showed that sixty-one chromatogram peaks from essential oil of pomelo leaf were isolated,thirty-nine volatile compounds had been identified,which was accounted for 90.05% of total volatile compounds.The predominant volatile components were isospathulenol(21.36%),caryophyllene oxide(17.16%),β-caryophyllene(8.40%),α-copaene(2.07%),isoaromadendrene epoxide(2.01%).Forty-two chromatogram peaks from essential oil of pomelo flower were isolated,twenty-eight volatile compounds had been identified,which was accounted for 89.31% of total volatile compounds.The predominant volatile components were farnesol(19.02%),nerolidol(18.24%),borneol(6.60%),linalool(4.03%),nerol(2.57%).Twelve chemical components such as nerol,geraniol,geraniol acid,β-aryophyllene were both presented in pomelo leaf and flower,but with different contents.