乳铁蛋白对小鼠肠道微生物区系的影响研究

目的:分析灌胃乳铁蛋白对小鼠肠道内一些具有代表性的微生物种群的变化规律及其特征,进一步了解乳铁蛋白对肠道菌群结构的作用,为开发具有肠道健康维护的功能性食品提供科学依据。方法:将小鼠饲养一周后随机分为5组,对照组,安慰剂组,低、中、高乳铁蛋白组,对照组正常饮食,安慰剂组灌胃生理盐水0.2mL,实验组灌胃不同剂量(1、10、100mg/(kg·d))的乳铁蛋白溶液,连续喂养14d。利用传统培养技术分别对小鼠肠道粪便菌群进行测定,通过数据分析了解小鼠肠道菌群的变化。结果表明喂食乳铁蛋白(10mg/(kg·d))后小鼠肠道中乳酸菌,双歧杆菌数量显著升高(p<0.05);而肠道中的肠杆菌和肠球菌的数量明显降低(p<0.05)。说明一定剂量的乳铁蛋白(10mg/(kg·d))对肠道中的益生菌和常驻共生菌的生长具有显著的促进作用,而对肠杆菌,肠球菌等细菌均有一定的抑制作用。 

Influence of milk-derived lactoferrin on intestinal microbial flora in mice

Purpose: To learn more about the role of Lactoferrin on intestinal microflora and provide a scientific basis for the development of functional foods with intestinal health maintenance, the changes and characteristics of some representative microbial populations were investigated, which exist in mice intestine when orally administered with Lactoferrin.Method: After having been fed for a week, mice were randomly divided into five groups.Each mouse in control group was fed normally, placebo group was given normal saline 0.2mL/ (kg·d) , low-dose group was given lactoferrin 1mg/ (kg·d) , middle-dose group was given lactoferrin 10mg/ (kg·d) and high-dose group was given lactoferrin 100mg/ (kg·d) respectively for 14d. Fecal flora in every group of mice were measured by traditional culture techniques, and the changes of intestinal flora in mice were analyzed. Results indicated that during lactoferrin groups (10mg/ (kg·d ) ) in mice, Lactobacillus and Bifidobacterium significantly increased compared with that in the normal control (p < 0.05) ; while the number of Enterobacteriaceae and Enterococci in mice intestinal tract decreased significantly (p < 0.05) .Conclusions: The experiment demonstrated that a certain dose of lactoferrin ( 10mg/ (kg·d) had a benefit on promoting the growth of intestinal probiotics and conventional symbiotic bacteria, whereas for Enterobacteriaceae and Enterococci etc.had certain inhibitory effect.