| 缬氨酸转氨酶拆分DL-缬氨酸的催化条件 |
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| 引用本文： | 张飞,魏涛,刘寅,韩亚伟,何培新.缬氨酸转氨酶拆分DL-缬氨酸的催化条件[J].食品与发酵工业,2013,39(2):41-44. |
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| 作者姓名： | 张飞 魏涛 刘寅 韩亚伟 何培新 |
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| 作者单位： | 郑州轻工业学院,食品与生物工程学院,河南郑州,450002 |
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| 基金项目： | 郑州轻工业学院博士基金资助项目 |
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| 摘 要： | 利用具有缬氨酸转氨酶活性的工程菌对DL-缬氨酸进行拆分,考察了反应温度、pH值、底物摩尔比、底物浓度和金属离子对酶活性和底物转化率的影响。结果显示,该催化反应的最适反应条件为:反应温度是45℃,pH=9,L-缬氨酸与丙酮酸的摩尔比1∶8,DL-缬氨酸初始浓度为0.6 mol/L、丙酮酸初始浓度为2.4 mol/L,0.5 mmol/L的Mg2+和Na+对酶活性有明显的促进作用。
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| 关 键 词： | 缬氨酸 转氨酶 拆分 |
Study on chiral resolution of DL-valine valine catalyzed by valine-pyruvate transaminase |
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| Zhang Fei,Wei Tao,Liu Yin,Han Ya-wei,He Pei-xin.Study on chiral resolution of DL-valine valine catalyzed by valine-pyruvate transaminase[J].Food and Fermentation Industries,2013,39(2):41-44. |
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| Authors: | Zhang Fei Wei Tao Liu Yin Han Ya-wei He Pei-xin |
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| Affiliation: | (School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China) |
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| Abstract: | An engineered strain with catalytic activity of valine-pyruvate transaminase was used in chiral resolution of DL-valine.The effect of reaction conditions such as reaction temperature,pH,substrate mole ratio,substrate concentration and metal ions on enzyme activity was studied.Results showed that the optimal conditions were 45℃ and pH9.The optimal substrate ratio of L-valine to pyruvic acid was 1∶ 8(mol∶ mol).The initial substrate concentration of DL-valine and pyruvic acid were 0.6 mol/L and 2.4 mol/L respectively.Enzyme activity was improved by addition of 0.5 mmol/L Mg2+ and Na+. |
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| Keywords: | valine transaminase chiral resolution |
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