云南栽培玛咖总生物碱提取工艺优化研究

以云南栽培玛咖干粉为原料,对其中总生物碱的提取工艺进行优化。采用热水为提取溶剂,以提取温度、提取时间和料液比为考察对象,在单因素优化的基础上设计3因素3水平正交实验,研究玛咖总生物碱的最优提取条件。结果表明,各因素对玛咖总生物碱提取得率的影响顺序依次为:提取时间>料液比>提取温度。最优提取工艺为:料液比1:20(g:mL),温度70℃,时间100min,在此条件下,玛咖总生物碱提取得率为1.58%。 

Study on optimization of extraction of total alkaloids from maca(Lepidium meyenii Walp.) cultivated in Yunnan

Extraction process of total alkaloids from maca (Lepidium meyenii walp.) cultivated in Yunnan was investigated. With hot water as extraction solvent, the effect of extraction temperature, extraction time and solid -liquid ratio on the yield of total alkaloids were optimized and the best extraction conditions were determined with single factor test followed by three factor-three level orthogonal experiment. The results showed that the effect of the investigated factors on the total alkaloids extraction yield was in the order as:extraction time>solid-liquid ratio>extraction temperature. The optimal extraction conditions were that solid-liquid ratio of 1:20 (g:mL) , extraction temperature of 70℃ and extraction time of 100min. Under these conditions the total alkaloids extraction yield was 1. 58%.