超临界CO_2法萃取芹菜叶黄酮

在考察超临界CO2萃取温度、萃取压力、萃取时间以及夹带剂用量等单因素对黄酮萃取率影响的基础上,采用Box-Behnken响应曲面设计法,建立影响因素和黄酮得率之间的回归方程。结果表明:萃取温度、萃取压力、萃取时间对超临界CO2萃取芹菜中黄酮工艺影响极显著,夹带剂用量影响显著,最佳提取工艺条件为萃取温度70℃、萃取压力30 MPa、萃取时间2.5 h、夹带剂用量为1 mL/g。该条件下黄酮得率为9.03%,与预测值相近,差异不显著,符合实验要求。

Optimization of flavonoids extraltion from Opium graveolens L by supercritical CO2

In the extraction of flavonoids from Opium graveolens L.with supercritical CO2,four operating parameters such as extractive pressure,temperature,time and cosolvent dosage that affect extraction yield of flavonoids were investigated.Box-Behnken response surface methodology design was applied to establish a regression model describing the relationship between the extraction efficiency and four operating parameters.The results showed that supercritical CO2 extraction process were impacted significantly by extracting pressure,temperature and extracting time.The optimum extraction conditions were: extraction temperature 70℃,extraction pressure 30 MPa,extraction time 2.5 h and dosage of alcohol 1 mL/g.Under these conditions,the average yield of flavonoids in three replicated experiments could reach 9.03%.which was very close to the predicted.