DNA damage-induced by sodium flouride (NaF) and the effect of cholicalciferol

It is known that the high electronegativity of fluorine affects various soft tissues, especially the bone structure inorganisms. Of these tissues are the kidneys, which play an important role in the excretion of fluoride from the body.Fluoride affects many cellular mechanisms. One of these effects is DNA damage. Our study aimed to investigate thelikely protective effect of cholecalciferol (vitamin D3) on genomic DNA damage-induced NaF depending onconcentration and time. The IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase inproliferation to vitamin D₃ through MTT assay in NRK-52E kidney cells were determined. DNA damage wassignificantly increased (p < 0.05) compared to the control group in all groups except for vitamin D₃. It wasdetermined that treatment with NaF together with vitamin D₃ decreased the DNA damage compared to NaF treatedgroups for 3 and 12 h. NaF combined with vitamin D3 was determined statistically to decrease (p < 0.05) DNAdamage compared to NaF treated groups for 24 h. As a result, it was determined that the treatment with cytotoxicconcentration NaF depending on the time significantly increased (p < 0.05) the genomic DNA damage, but NaFtreatment together with vitamin D₃ decreased the DNA damage in renal cells depending on the time. It wasconcluded that vitamin D₃ may be useful in preventing DNA damage caused by NaF.