酶电极法快速测定加热卷烟中的甘油含量

  目的  快速准确测定加热卷烟中的甘油含量。  方法  建立了基于生物传感器的酶电极分析法，检测结果与气相色谱法进行了对比分析，对加热卷烟抽吸过程甘油的转移率进行测定。  结果  结果表明:（1）酶电极法测定甘油的标准工作曲线线性关系良好（R2=0.9998），检出限和定量限分别为0.045 mg/mL、0.149 mg/mL，加标回收率为97.64%~102.32%，RSD为2.15%（n=6），重复性良好。（2）酶电极法与气相色谱法两种分析方法的测定结果之间的差异无统计学意义。（3）加热卷烟抽吸过程中甘油的转移率为33%~41%。  结论  与气相色谱法相比，酶电极法具有酶专一性高、稳定性好、检测快速灵敏等特点，适合于加热卷烟及加热卷烟用烟芯材料中甘油含量的快速检测。 

Rapid determination of glycerol in HTP cigarette with enzyme electrode method

  Objective  This study aims to realize rapid and accurate determination of glycerol content in HTP cigarette.  Methods  Enzyme electrode method based on biosensors was established, and the detection results from enzyme electrode method were compared with those of GC method. The transfer rate of glycerol during smoking was also measured.  Results  (1) There was a significant linear correlation (R2=0.9998) in standard curves of enzyme electrode method, and the LOD, LOQ, recovery rate and RSD were 0.045mg/mL, 0.149 mg/mL, 97.64%~102.32%, and 2.15% (n=6), respectively, showing improved repeatability. (2) There was no significant difference between the test results from enzyme electrode method and those from GC method, and the accuracy of the established method was better. (3)The transfer rate of glycerol during smoking process was within the range from 33% to 41%.  Conclusion  Compared with GC method, the enzyme electrode method has high specificity, high stability, fast detection and high sensitivity, which can be suitably applied in determining the amount of glycerol in heat-not-burn cigarette and corresponding tobacco material.