Effect of Black Sea bream extracts on cytokine production in lipopolysaccharide-induced inflammation

We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatorycytokines induced by lipopolysaccharide (LPS) in murine splenocytes. The levels of pro-inflammatory cytokines[interleukin (IL)-6, tumor necrosis factor-α (TNF-α), IL-12/IL-23(p40) and IL-17A] and anti-inflammatory cytokines[IL-4, IL-10 and interferon-γ (IFN-γ)] were assessed. Incubation of murine splenocytes with acetone+methylenechloride (A+M) and methanol (MeOH) extracts significantly decreased LPS-induced IL-6, IL-12/IL-23(p40) andIL-17A productions after 6 h incubation (p < 0.05). The A+M and MeOH extracts significantly increased LPS-inducedIL-4 and IFN-γ productions at 48 and 72 h incubation (p < 0.05). Treatment with A+M extract resulted insignificantly higher IL-10 production in splenocytes after 72 h (p < 0.05). Conclusively, black sea bream extracts wereshown to be efficient in falling several pro-inflammatory cytokines while rising anti-inflammatory cytokines. Thus ourresults suggest that black sea bream extracts selectivity modulate immune events.