| Abstract: | Osteosarcoma (OS) is a primary high-grade malignant bone neoplasm, and the prognosis of OS remains poor due toearly metastasis. Leptin plays an essential role in tumorigenesis, but the role of leptin in the development of OS is still not fullyunderstood. In this study, we used a human osteosarcoma MG-63 cell line as an experimental model. MG-63 cells were treatedwith leptin, and cell proliferation, apoptosis, adhesion, invasion, and gene expression, were evaluated. The results showed thatleptin promoted proliferation, decreased adhesion, suppressed apoptosis, and promoted invasion, of MG-63 cells. Moreover,the expression of SIRT1 was upregulated in MG-63 cells exposed to leptin. Furthermore, MMP-2, 8, and 9 were significantlyupregulated by SIRT1, while SIRT1 knockdown inhibited the proliferation and invasion of MG-63 cells. In conclusion, ourresults suggest that leptin promotes OS cell proliferation and invasion by inducing the expression of SIRT1. |
