| Abstract: | Randomly amplified polymorphic DNA (RAPD) is a tremendously convenient approach used to discriminatebetween Brassica species owing to its accuracy and speed. RAPD primers generate adequate genetic information that canbe used in the primer-marker system. In this work, twenty RAPD-PCR based markers were executed to generatepolymorphic data, like polymorphic information content (PIC), mean resolving power (MRP), resolving power (RP),effective multiplex ratio (EMR), and marker index (MI) for the first time and genetic distance among and between sixBrassica species were calculated. Our results indicated that 20 primers produced a total of 231 scored band andgenerated 87% polymorphic bands. Average PIC, MRP, RP, MI, and EMR values were 0.088, 0.65, 6.7, 0.78, and 8.9,respectively. PIC showed an overall negative correlation with MRP, RP, MI, and EMR, whereas MRP, RP, and EMR,were positively correlated with each other. Genetic identities ranged from 41.99% (between Brassica napus andBrassica oleracea) to 68.83% (between Brassica campestris and Brassica oleracea). Dendrogram results showed noclustering between species except between Brassica campestris and Brassica nigra. Nevertheless, these results will behelpful to acquire useful information about the markers and their use to determine the genomic structures of Brassicaspecies. Further, based on genetic distance and polymorphic information, new hybrids can be developed for effectiveoilseed production. |
