| 黄芩愈伤组织与丛芽诱导 |
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| 引用本文： | 刘娟,;徐蓓蕾,;张跃华. 黄芩愈伤组织与丛芽诱导[J]. 佳木斯工学院学报, 2008, 0(6): 856-858 |
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| 作者姓名： | 刘娟, 徐蓓蕾, 张跃华 |
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| 作者单位： | [1]佳木斯大学化学与药学院,黑龙江佳木斯154007; [2]佳木斯大学生命科学院,黑龙江佳木斯154007 |
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| 摘 要： | 为寻求黄芩愈伤组织、丛芽诱导的最佳条件,分别以茎尖、茎段、叶片、带节茎段等为实验材料比较不同诱导条件对诱导率的影响;比较培养过程中愈伤组织的增重量及生长状态.结果表明,诱导率：带节茎段要高于其它外植体;MS＋6-BA0.5 mg/L＋NAA0.5 mg/L为愈伤组织诱导率最高、愈伤组织增殖最大的培养基组合;MS＋6-BA1.0 mg/L＋NAA0.5 mg/L为不定芽诱导最合适培养基.
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| 关 键 词： | 黄芩 愈伤组织 诱导率 增重量 丛芽 |
Study on Callus and Clumpy Buds Induction of Scutellaria Baicalensis Georgi |
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| Affiliation: | LIU Juan, XU Bei - lei, ZhANG Yue - hua(1.The chemistry and pharmacy college of Jianmusi University,Jiamusi 154007,China;2.The academy for life science of Jiamusi university.Jianmusi 154007,China) |
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| Abstract: | To find the optimum conditions of callus and clumpy buds induction of Scutellaria baicalenis Georgi, different induction rate effects were compared with different inducing conditions of shoot tip, stem , leaf, and stem -segment with node. The increased weight of callus induction and growth status in cultivation process was compared as well. The results showed that the best induction rate is stem - segment with node, MS ＋ 6 - BA0. 5 mg/L ＋ NAA0. 5 mg/L is the optimum medium combinations of the best induction rate and increased weight, and MS ＋ 6 - BA1.0 mg/ L ＋ NAA0.5 mg/L is suitable for inducing adventitious buds. |
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| Keywords: | Scutellaria baicahnsis Georgi Calus induction induction rate increased weight clumpy buds |
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