微生物发酵法批量制备高丰度~(15)N同位素标记L-精氨酸

采用均匀设计实验方法建立5L罐发酵生产L-精氨酸-~(15)N_4的优化调控模型。在菌体生长24h进入产酸阶段,控制转速820r/min,pH7.8,温度30℃,通气2.5L/min,流加总量60%的葡萄糖,理论产酸最高可达到22.49g/L,验证实验产酸19.69g/L,达到理论高值的88%。利用~(15)N丰度99.68%的尿素和丰度99.43%的硫酸铵发酵生产得到L-精氨酸-~(15)N_4的产品丰度为98.7%,化学纯度为98.5%,丰度下降1%。可以满足批量生产的要求。

Bath Production of High Abundance 15 N Isotope Labeled L-arginine by Microbiological Fermentation

Using uniform design,the optimal control model had been established for the fermentation of L-Arginine-15N4 in 5 L fermentation tank.L-arginine was produced when the cell grew about 24 h.The theoretical yield can up to 22.49 g/L when production condition controlled in 820 r/min,pH 7.8,temperature in 30 ℃,the ventilation 2.5 L/min and fed-batch 60％ glucose.While the actual yield was 88％ of theoretical yield and 19.69 g/L of L-Arginine,which was verified.L-Arginine-15N4 was produced by the abundance 99.68％ of urea and 99.43％ of ammonium sulphate,which 15N isotopic abundance was up to 98.7％,the purity was 98.5％.The 15N isotope abundance decreased less than 1％.The process was satisfied the request of batch production.