黄粉虫幼虫蛋白质的提取研究

研究了不同的介质pH值、提取时间、提取温度和加水比例对黄粉虫幼虫蛋白质的可溶出率(提取率)的影响,分析了3种不同pH值提取的蛋白质的氨基酸组成。结果表明,pH值极大地影响着黄粉虫幼虫蛋白质的溶出率,pH值5.8时溶出率最低,此时可能为黄粉虫蛋白的等电点;pH值10.0或高于此值具有较高的溶出率;水与虫之比为10时蛋白质的可溶出率较其它比例为高;其它的因素对幼虫蛋白质的溶出率影响不大。在pH值2.4、5.8和12.1下提取的蛋白质其氨基酸组成不同,提取的黄粉虫蛋白质必需氨基酸占总氨基酸的含量达40％以上。

Study on The Extractablity of Protein from Yellow Mealworm Tenebrio molitor(L. ) Larvae

The effect of pH value, extracting time, extracting temperature, ratio of extracting medium to material was studied. The amino acid profile of extracted protein at three pH value was analysed by the HPLC. The results showed that pH value greatly influenced the solubility, a minimum solubility was found at pH 5. 8. The solubility increased as the pH value increases . Higher protein solubiltiy was get at the ratio of water to larva 10. It was observed that Other factors not siginificantly affected the solubility of the protein. Lipuid protein ectracted at different pH medium contained different kinds of amino acid . The ratio of essential amino acid to total amino acid was more than 40% in all samples. It suggested that the protein extracted from yellow mealworm larvae was a high quality protein.