Demonstration of chicken B alloantigens by the Cytotoxicity test

The activity of complements from various sources was compared in the complement-mediated cytotoxicity test, using chicken alloantisera. The highest cytotoxicity index was obtained with a pool of complements from fresh guinea pig serum and chicken serum. No difference in activity between complements from chickens of the different inbred lines was found. Using the above test, we demonstrated the B-F antigen on lymphoid cells, whereas the B-G antigen was not present.