Prenylated Flavonoids from Cudrania tricuspidata Suppress Lipopolysaccharide-Induced Neuroinflammatory Activities in BV2 Microglial Cells |
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| Authors: | Dong-Cheol Kim Chi-Su Yoon Tran Hong Quang Wonmin Ko Jong-Su Kim Hyuncheol Oh Youn-Chul Kim |
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| Affiliation: | 1.Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570–749, Korea; (D.-C.K.); (C.-S.Y.); (T.H.Q.); (W.K.); (J.-S.K.); (H.O.);2.Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi 10000, Vietnam |
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| Abstract: | In Korea and China, Cudrania tricuspidata Bureau (Moraceae) is an important traditional medicinal plant used to treat lumbago, hemoptysis, and contusions. The C. tricuspidata methanol extract suppressed both production of NO and PGE2 in BV2 microglial cells. Cudraflavanone D (1), isolated from this extract, remarkably suppressed the protein expression of inducible NO synthase and cyclooxygenase-2, and decreased the levels of NO and PGE2 in BV2 microglial cells exposed to lipopolysaccharide. Cudraflavanone D (1) also decreased IL-6, TNF-α, IL-12, and IL-1β production, blocked nuclear translocation of NF-κB heterodimers (p50 and p65) by interrupting the degradation and phosphorylation of inhibitor of IκB-α, and inhibited NF-κB binding. In addition, cudraflavanone D (1) suppressed the phosphorylation of c-Jun N-terminal kinase (JNK) and p38 MAPK pathways. This study indicated that cudraflavanone D (1) can be a potential drug candidate for the cure of neuroinflammation. |
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| Keywords: | Cudrania tricuspidata cudraflavanone D microglia neuroinflammation nuclear factor-κ B mitogen-activated protein kinase |
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