| 麦冬多糖糖苷酶的分离纯化及其酶性质 |
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| 引用本文： | 薛冬令,韩祥,金凤燮,鱼红闪. 麦冬多糖糖苷酶的分离纯化及其酶性质[J]. 大连工业大学学报, 2009, 28(4) |
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| 作者姓名： | 薛冬令 韩祥 金凤燮 鱼红闪 |
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| 作者单位： | 大连工业大学,生物与食品工程学院,辽宁,大连,116034 |
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| 基金项目： | 国家自然科学基金资助项目，辽宁省高等学校优秀人才支持计划资助项目，辽宁省科学技术基金资助项目 |
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| 摘 要： | 分离纯化了Absidia sp.O84s菌产的麦冬多糖糖苷酶,并对其酶性质进行了研究。结果表明,酶蛋白的分子质量为72 ku,最适pH为5.0,在pH 4.0~8.0范围内稳定。最适反应温度为40℃,在20~55℃范围内稳定。Ca+、K+、Na+、Fe3+、Mg2+Zn2+对该酶活性没有影响,Cu2+对酶活力具有抑制作用。酶反应动力学参数Vmax=0.131 5 mmol/(h.L),Km=2.375 mmol/L。
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| 关 键 词： | 麦冬多糖 糖苷酶 酶性质 |
Purification and characterization of Ophiopogon japonicus polysaccharide glycosidase |
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| XUE Dong-ling,HAN Xiang,JIN Feng-xie,YU Hong-shan. Purification and characterization of Ophiopogon japonicus polysaccharide glycosidase[J]. Journal of Dalian Dalian Polytechnic University, 2009, 28(4) |
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| Authors: | XUE Dong-ling HAN Xiang JIN Feng-xie YU Hong-shan |
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| Abstract: | Polysaccharide glycosidase was purified and characterized from Absidia sp.O84s.Its molecular weight was 72 ku,and the optimum pH and temperature was pH 5.0 and 40 ℃.The glycosidase was stable at pH 4.0-8.0 and 20-50 ℃ respectively.Ions K+,Zn2+,Na+,Mg2+,Ca2+ had no effect on the enzyme activity and Cu2+ decreased enzyme activity.The kinetic analysis revealed that Vmax was 0.131 5 mmol/(h·L) and Km was 2.375 mmol/L. |
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| Keywords: | Ophiopogon japonicus polysaccharide glycosidase characterization |
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