Rapid Direct Extraction Derivatization Method for the Determination of Acylglycerol Lipids in Selected Sample Matrices |
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| Authors: | AUSTIN R. LONG STEVEN J. MASSIE WILLIAM J. TYZNIK |
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| Affiliation: | Author Long is with the Dept. of Food Science &Nutrition, the Ohio Agricultural Research &Development Center, Columbus, OH 43210. Authors Massie and Tyznik are with the Dept. of Animal Science, The Ohio State Univ., Columbus, OH 43210. |
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| Abstract: | Acylglycerol lipids as total fatty acid methylesters (FAME) were rapidly determined by direct extraction derivatization from sample matrices utilizing 0.4N methanolic sodium methoxide in hexane solution and quantitated using gas chromatography. FAME values determined by the direct extraction derivatization (DED) method and standard AOAC ether extraction saponification method were similar for all samples. The DED method was reproducible and required less than 35 min total sample preparation and analysis time compared to a minimum of 6 hr needed for the standard AOAC method. The DED method yielded FAME derivatives of acylglycerol lipids which were free from interfering compounds when analyzed by gas chromatography. Identity and purity of isolated FAME were positively confirmed by mass spectrometry by comparison with authentic standards. |
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