Protein Extraction From Chicken Myofibrils Irrigated with Various Polyphosphate and NaCl Solutions |
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Authors: | YL Xiong X Lou C Wang WG Moody RJ Harmon |
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Affiliation: | Authors Xiong, Moody, and Harmon are with the Department of Animal Sciences, University of Kentucky, Lexington, KY 40546.;Author Lou is now with AMPC, Inc., Ames, IA 50010.;Author Wang is with Human Nutrition Program, Kentucky State University, Frankfort, KY 40601. |
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Abstract: | Physical changes in chicken gastrocnemius myofibrils incubated in 0.1 to 1.0 M NaCl solutions with or without 10 mM ortho-(P), pyro-(PP), tripoly-(TPP) or hexameta- (HMP) phosphate at pH 6.0 were examined by phase-contrast microscopy, electrophoresis, and solubility. PP and TPP performed similarly in promoting protein extraction, P had no apparent effect, and HMP exhibited an intermediate effect. PP, TPP, and HMP treatments markedly improved protein solubility in 0.3 and 0.4 M NaCl through the release of myosin, but the phosphate effect diminished in ≥ 0.6 M NaCl. Overall, phosphates influenced the ultrastructure of myofibrils and extraction of their constituents in the order: PP ∼ TPP > HMP > P ∼ nonphosphate control. |
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Keywords: | myofibril protein extraction polyphosphate phase contrast microscopy |
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