序批式MBBR中反硝化聚磷菌的选择与富集

采用序批式移动床生物膜反应器(MBBR),研究了以硝酸盐为电子受体的反硝化聚磷菌(DPB)的选择和富集.结果表明,采用3个阶段进行选择和富集,DPB占全部聚磷菌(PAOs)比例约从11.77%提高到66.07%;第3阶段培养末期,COD和TP去除率平均值分别为68.78%和69.02%,缺氧所耗ρ(NO3--N)达到23.91 mg/L;对反应器中生物膜进行直接染色发现,在厌氧放磷阶段能观察到聚磷菌体内有大量聚-β-羟丁酸(PHB)出现,而在缺氧吸磷阶段则有大量聚磷颗粒(Poly-p)出现,故可尝试采用聚磷生物膜的直接染色方法观察聚磷微生物细胞内PHB和Poly-p颗粒的变化,来判断生物除磷过程及效果.

Selection and Enrichment of Denitrifying Phosphate Removing Bacteria in Moving-bed Biofilm Reactor

The selection and dominance of denitrifying phosphorus removing bacteria(DPB),which was capable of utilizing nitrate as electron acceptor,was investigated in a laboratory-scale sequencing batch moving-bed biofilm reactor(MBBR).The experimental results demonstrated that the proportion of DPB increased approximately from 11.77% to 66.07% of total phosphate accumulating organisms(PAOs) after three stages selection and dominance.In the end of the third cultivating period,average removal efficiencies of COD and TP were 68.78% and 69.02%,respectively,and a large quantity of nitrate,23.91 mg/L,was removed in anoxic phase.It was found that a great deal of PHB about PAOs emerged in anaerobic phase,whereas a mass of Poly-p about PAOs arose in anoxic phase by biofilm dyeing method,so they could be taken as evidences to judge the process and efficiency of biological phosphorus removal.