The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli |
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Authors: | Wall JGerard; Pluckthun Andreas |
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Affiliation: | 1 Biochemisches Institut der Universität Zürich, Winterthurerstrasse 190,CH-8057 Zürich, Switzerland and
2 Industrial Biochemistry Group, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland |
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Abstract: | In a systematic study of the periplasmic folding of antibodyfragments in Escherichia coli, we have analysed the expressionof an aggregation-prone and previously non-functional anti-phosphorylcholineantibody, T15, as a model system and converted it to a functionalmolecule. Introduction of heavy chain framework mutations previouslyfound to improve the folding of a related antibody led to improvedfolding of T15 fragments and improved physiology of the hostE.coli cells. Manipulation of the complementarity determiningregions (CDR) of the framework-mutated forms of T15 furtherimproved folding and bacterial host physiology, but no improvementwas seen in the wild type, suggesting the existence of a hierarchyin sequence positions leading to aggregation. Rational mutagenesisof the T15 light chain led to the production of functional T15fragments for the first time, with increased levels of functionalprotein produced from VH manipulated constructs. We proposethat a hierarchical analysis of the primary amino acid sequence,as we have described, provides guidelines on how correctly folding,functional antibodies might be achieved and will allow furtherdelineation of the decisive structural factors and pathwaysfavouring protein aggregation. |
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Keywords: | antibody engineering / protein folding/ protein misfolding/ protein aggregation/ rational design |
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