Lipase-catalyzed acidolysis of olive oil and caprylic acid in a bench-scale packed bed bioreactor |
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| Affiliation: | 1. Escuela de Química, Grupo de investigación en Bioquímica y Microbiología (GIBIM), Edificio Camilo Torres 210, Universidad Industrial de Santander, CEP, 680001 Bucaramanga, Colombia;2. ICP-CSIC Campus UAM-CSIC, Cantoblanco, 28049 Madrid, Spain;3. Departamento de Engenharia Química, Universidade Federal Do Ceará, Campus Do Pici, CEP, 60455-760 Fortaleza, CE, Brazil;4. Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia;5. Departamento de Química, Facultad de Ciencias. Universidad del Tolima, Ibagué, Colombia;1. Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;2. College of Life Science, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China;3. Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China;1. Department of Oils, Oleochemicals and Surfactant Technology, Institute of Chemical Technology, Matunga, Mumbai 400019, India;2. Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 400019, India;3. Department of Rural Development, Indian Institute of Technology, Delhi 110016, India;2. Univ Lyon, Université Lyon 1, INSA Lyon, CPE Lyon, Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires, UMR 5246, Chimie Organique et Bioorganique, Bâtiment Jules Verne, 20 Avenue Albert Einstein, 69621 Villeurbanne Cedex, France |
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| Abstract: | Lipase-catalyzed acidolysis of olive oil and caprylic acid was performed in a bench-scale packed bed bioreactor to produce structured lipids (SL). A 1,3-specific lipase, IM 60 from Rhizomucor miehei was used as the biocatalyst. Reaction products were analyzed by reversed phase high-performance liquid chromatography, with an evaporative light scattering detector. Olive oil is characterized by four major clusters of triacylglycerol species with equivalent carbon number (ECN), C44, C46, C48, and C50. Three monosubstituted products and two disubstituted products were detected after the reaction. Monosubstituted products had ECN of C36, C38, and C40, and disubstituted products had ECN of C30 and C32. The effect of solvent, temperature, substrate mol ratio, and flow rate/residence time were studied. Optimal solvent-free production of SL was obtained at a substrate flow rate of 1 ml/min, residence time 2.7 h, temperature 60°C, and mol ratio 1:5 (olive oil/caprylic acid). Fatty acid distribution at the sn-2 position of olive oil was determined by pancreatic lipase hydrolysis as 74.8% oleic acid and 25.2% linoleic acid. SL produced at optimal conditions had 7.2% caprylic acid, 69.6% oleic acid, 21.7% linoleic acid and 1.5% palmitic acid at the sn-2 position. |
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