Structure and Biosynthesis of Fimsbactins A–F,Siderophores from Acinetobacter baumannii and Acinetobacter baylyi |
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| Authors: | Anna Proschak Patrice Lubuta Peter Grün Dr. Frank Löhr Dr. Gottfried Wilharm Veronique De Berardinis Prof. Dr. Helge B. Bode |
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| Affiliation: | 1. Fachbereich Biowissenschaften, Goethe University Frankfurt, Max‐von‐Laue‐Strasse 9, 60438 Frankfurt am Main (Germany);2. Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max‐von‐Laue Strasse 9, 60438 Frankfurt am Main (Germany);3. Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, 38855 Wernigerode (Germany);4. CEA, DSV, IG, Genoscope and CNRS‐UMR8030, 2 rue Gaston Crémieux, 91057 Evry (France);5. Université d'Evry Val d'Essonne, Boulevard Fran?ois Mitterrand, 91057 Evry (France) |
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| Abstract: | Novel chatechol/hydroxamate siderophores (named “fimsbactins”) were identified in Acinetobacter baumannii ATCC 17978 and Acinetobacter baylyi ADP1. The major compound, fimsbactin A, was isolated from low‐iron cultures of A. baylyi ADP1, and its chemical structure was elucidated by mass spectrometry, and detailed 1H, 13C and 15N NMR spectroscopy. From inverse feeding experiments following HPLC‐MS analysis, the structures of five additional derivatives were elucidated. The gene cluster encoding the fimsbactin synthetase (fbs) was identified in both genomes, and mutants in fbs genes in A. baylyi were analyzed, thus allowing prediction of the fimsbactin biosynthesis pathway. |
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| Keywords: | Acinetobacter baumannii enzymes natural products nonribosomal peptide synthetases secondary metabolites siderophores |
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