Resin Acid Conversion with CYP105A1: An Enzyme with Potential for the Production of Pharmaceutically Relevant Diterpenoids |
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| Authors: | Simon Janocha Dr Josef Zapp Dr Michael Hutter Dr Michael Kleser Prof Dr Jörg Bohlmann Prof Dr Rita Bernhardt |
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| Affiliation: | 1. Universit?t des Saarlandes, Biochemie, Campus B2.2, 66123 Saarbrücken (Germany);2. Universit?t des Saarlandes, Pharmazeutische Biologie, Campus C2.2, 66123 Saarbrücken (Germany);3. Universit?t des Saarlandes, Center for Bioinformatics, Campus E2.1, 66123 Saarbrücken (Germany);4. Michael Smith Laboratories, University of British Columbia, 321‐2185 East Mall, Vancouver, BC V6T 1Z4 (Canada) |
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| Abstract: | Cytochrome P450s are very versatile enzymes with great potential for biotechnological applications because of their ability to oxidize unactivated C? H bonds. CYP105A1 from Streptomyces griseolus was first described as a herbicide‐inducible sulfonylurea hydroxylase, but it is also able to convert other substrates such as vitamin D3. To extend the substrate pool of this interesting enzyme further, we screened a small diterpenoid compound library and were able to show the conversion of several resin acids. Binding of abietic acid, dehydroabietic acid, and isopimaric acid to the active site was assayed, and Vmax and Km values were calculated. The products were analyzed by NMR spectroscopy and identified as 15‐hydroxyabietic acid, 15‐hydroxydehydroabietic acid, and 15,16‐epoxyisopimaric acid. As the observed products are difficult to obtain by chemical synthesis, CYP105A1 has proved to be a promising candidate for biotechnological applications that combine bioconversion and chemical synthesis to obtain functionalized resin acids. |
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| Keywords: | 15‐hydroxyabietic acid biocatalysis cytochromes P450 hydroxylation terpenoids |
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