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Nucleobase Protection Strategy for Gene Cloning and Expression
Authors:Pavel Kielkowski  Nelson L. Brock  Dr. Jeroen S. Dickschat  Prof. Dr. Michal Hocek
Affiliation:1. Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, 16610 Prague 6 (Czech Republic) http://www.uochb.cas.cz/hocekgroup;2. Institut für Organische Chemie, Technische Universit?t Braunschweig, Hagenring 30, 38106 Braunschweig (Germany) http://www.oc.tu‐bs.de/dickschat/startseite_de.html;3. Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 12843 Prague 2 (Czech Republic)
Abstract:
Keywords:DNA  polymerase chain reaction  protecting groups  protein production  restriction endonucleases
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