| 微滴式数字聚合酶链式反应在食品安全检测领域的应用 |
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| 引用本文： | 牛会敏,王静怡,姚晓洁,魏华琳,陈万胜,邓迎春. 微滴式数字聚合酶链式反应在食品安全检测领域的应用[J]. 食品安全质量检测学报, 2020, 11(24): 9295-9300 |
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| 作者姓名： | 牛会敏 王静怡 姚晓洁 魏华琳 陈万胜 邓迎春 |
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| 作者单位： | 河南省口岸食品检验检测所,河南省口岸食品检验检测所,河南省产品质量监督检验院,河南省口岸食品检验检测所,河南省口岸食品检验检测所,河南省口岸食品检验检测所 |
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| 基金项目： | 2020年度河南省市场监督管理局科技计划项目(2020sj39)、河南省科技攻关计划项目(172102310347)、河南省科技攻关计划项目(182102110384)、河南省科技攻关计划项目(202102210193) |
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| 摘 要： | 微滴式数字聚合酶链式反应(droplet digital polymerase chain reaction, ddPCR)是一种新型核酸扩增技术，可对DNA或RNA分子采用绝对定量的方式进行分析。其结果具有更高的精准度、准确性和灵敏度，大大提升了数字PCR技术的可扩展性与实用性，促进了现代分子生物学在精准定量检测方面的发展和应用。本文重点论述了ddPCR法的技术原理、优势以及在食源性致病微生物定量检测、转基因成分分析、食品源性成分检测等食品安全检测领域的应用研究进展情况。
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| 关 键 词： | 微滴式数字聚合酶链式反应 绝对定量 食品安全检测 |
| 收稿时间： | 2020-07-23 |
| 修稿时间： | 2020-10-09 |
Application of droplet digital polymerase chain reaction in food safety detection |
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| NIU Hui-Min,WANG Jing-Yi,YAO Xiao-Jie,WEI Hua-Lin,CHEN Wan-Sheng,DENG Ying-Chun. Application of droplet digital polymerase chain reaction in food safety detection[J]. Journal of Food Safety & Quality, 2020, 11(24): 9295-9300 |
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| Authors: | NIU Hui-Min WANG Jing-Yi YAO Xiao-Jie WEI Hua-Lin CHEN Wan-Sheng DENG Ying-Chun |
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| Affiliation: | Food inspection and Testing Institute of Henan Province,Food inspection and Testing Institute of Henan Province,Henan Institute of Product Quality Supervision an Inspection,Food inspection and Testing Institute of Henan Province,Food inspection and Testing Institute of Henan Province,Food inspection and Testing Institute of Henan Province |
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| Abstract: | Droplet digital polymerase chain reaction (ddPCR) is a new kind of nucleic acid amplification and technology, which can be used to analyze DNA or RNA molecules in an absolutely quantitative way. The result has higher precision, accuracy and sensitivity, which greatly enhance the scalability and practical of digital PCR technology, and promotes the development and application of modern molecular biology in accurate quantitative detection. The technical principle and advantages of ddPCR method and its application in food safety field, such as quantitative detection of food borne pathogenic microorganisms, analysis of transgenic components, detection of food-derived components were mainly reviewed in this paper. |
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| Keywords: | droplet digital polymerase chain reaction absolute quantification food safety detection |
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