实时荧光PCR法和国标法检测乳粉中单增李斯特菌的比较研究

目的 提升实验室检测食品中单核细胞增生李斯特氏菌的速度和准确性。方法 依据《GBS4789.30-2016S食品安全国家标准S食品微生物学检验S单核细胞增生李斯特氏菌检验》和能力验证作业指导书对食品中单核细胞增生李斯特氏菌进行检测, 同时利用实时荧光PCR法对能力验证中的2个样品进行快速筛查,采用VITEK2 Compact30全自动微生物鉴定系统鉴定可疑菌落。结果 GB 4789.30国标法和实时荧光PCR快速筛查法检测,结果均为样品CODE:FC02220028单核细胞增生李斯特氏菌阳性, 样品CODE:FC02220098阴性。结论 本实验室参加了中国食品药品质量检定研究院组织的食品中单核细胞增生李斯特氏菌检测能力验证,取得了满意的结果。

Comparative study for detection of Listeria monocytogenes by real-time fluorescent polymerase chain reaction and national standard method in milk powder

Objective To explore whether real-time fluorescent polymerase chain reaction(RT-PCR) method can be used to detect Listeria monocytogenes from milk powder in laboratory. Methods Listeria monocytogenes testing was carried out according to GB 4789.30-2016 National food safety standard-Food microbiological examination-Listeria monocytogenes and ability verification guidance book. RT-PCR method was used for testing the 2 samples, and suspicious strains of 2 samples were identified by automatic microbial identification system VITEK2 Compact 30. Then the results of national standard method and RT-PCR method were compared. Results Both national standard method and RT-PCR method proved that Listeria monocytogenes was detected in CODE: FC02220028, not detected in CODE: FC02220098. Conclusion RT-PCR method can be used to detect Listeria monocytogenes from milk powder in laboratory milk power.