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Biological evaluation of polyvinyl alcohol hydrogel crosslinked by polyurethane chain for cartilage tissue engineering in rabbit model |
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| Authors: | Mohammad Ali Shokrgozar Shahin Bonakdar Mohammad Mehdi Dehghan Shahriar Hojjati Emami Leila Montazeri Shahram Azari Mohsen Rabbani |
| Affiliation: | 1. National Cell Bank of Iran, Pasteur Institute of Iran, 13164, Tehran, Iran 2. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 3. Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran 4. Nano Medicine & Tissue Engineering Research Center, Ayatollah Taleghani Hospital, Parvaneh Avenue, Velenjak, Tehran, Iran |
| Abstract: | Polyvinyl alcohol (PVA) hydrogel chains were crosslinked by urethane pre-polymer (PPU) in order to fabricate a new substitute for cartilage lesions. The microscopy images showed that the cultured chondrocytes had spherical morphology on PVA–PPU sample after 4 weeks of isolation in vitro. The alcian blue and safranin O staining proved the presence of proteoglycan on the surface of PVA–PPU sample secreted by cultured chondrocytes. This was confirmed by the detection of sulfate ions in the wavelength dispersive X-ray (WDX) analysis. In addition, the expression of collagen type II and aggrecan were observed in chondrocytes cultured on PVA–PPU by RT-PCR. Moreover, the implantation of the PVA–PPU sample with autologous cultured chondrocytes revealed the formation of neocartilage tissue in a rabbit model during 12 weeks follow up. In conclusion, the results verified that isolated chondrocytes cultured on PVA–PPU retain their original phenotype and this composition can be considered as promising substrate for cartilage tissue engineering. |
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