| 用超热中子活化法测定大鼠肝脏亚细胞组分中的砷 |
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| 引用本文： | 杨瑞瑛,张永保,王生玲,张智勇,王珂,张玲,林勤,马品江,朱殿志,王连方. 用超热中子活化法测定大鼠肝脏亚细胞组分中的砷[J]. 核化学与放射化学, 2002, 24(3): 144-147 |
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| 作者姓名： | 杨瑞瑛 张永保 王生玲 张智勇 王珂 张玲 林勤 马品江 朱殿志 王连方 |
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| 作者单位： | 0253-9950(2002)03-0144-04 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9860 0 76) |
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| 摘 要： | 采用差速离心分离技术与超热中子活化分析法 (ENAA) ,测定了大鼠肝脏亚细胞组分 (细胞核、线粒体、溶酶体、微粒体、胞液 )中As的质量分数 ,并对标准参考物质作对照分析。分析结果表明 :As在各亚细胞组分中并非均匀分布。As在微粒体中的质量分数最高 ,在细胞核中最低。用t检验比较了在中毒组大鼠和正常组大鼠肝脏中各亚细胞组分中As含量的差别 ,以及中毒组大鼠肝亚细胞组分之间的差异。发现上述两组肝亚细胞组分中As存在极显著性差异 (P <0 0 0 1)。在中毒组肝亚细胞组分中 ,微粒体中As的含量与胞液无显著性差异 (P >0 0 5 ) ,但与细胞核、线粒体、溶酶体存在明显的差异 (P <0 0 5 )。
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| 关 键 词： | 大鼠肝脏 亚细胞组分 超热中子活化分析 砷 |
| 文章编号： | 0253-9950(2002)03-0144-04 |
| 修稿时间： | 2001-12-24 |
Determination of Arsenicin Subcellular Fractions of Rat Liver by ENAA |
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| YANG Rui ying ,ZHANG Yong bao ,WANG Sheng ling ,ZHANG Zhi yong ,WANG Ke ,ZHANG Ling ,LIN Qin ,MA Pin jiang ,ZHU Dian Zhi ,WANG Lian fang .Laboratory of Nuclear Analytical Techniques. Determination of Arsenicin Subcellular Fractions of Rat Liver by ENAA[J]. Journal of Nuclear and Radiochemistry, 2002, 24(3): 144-147 |
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| Authors: | YANG Rui ying ZHANG Yong bao WANG Sheng ling ZHANG Zhi yong WANG Ke ZHANG Ling LIN Qin MA Pin jiang ZHU Dian Zhi WANG Lian fang .Laboratory of Nuclear Analytical Techniques |
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| Affiliation: | YANG Rui ying 1,ZHANG Yong bao 2,WANG Sheng ling 3,ZHANG Zhi yong 1, WANG Ke 2,ZHANG Ling 3,LIN Qin 3,MA Pin jiang 3,ZHU Dian Zhi 3, WANG Lian fang 3 1.Laboratory of Nuclear Analytical Techniques,Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing 100039, China; 2.China Institute of Atomic Energy ,P.O.Box 275(49), Beijing 102413,China; 3.Xinjiang Institute for Prevention and Treatment of Endemic Disease, Wulumuqi 830002,China |
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| Abstract: | The concentration of arsenic in subcellular fractions of rat liver is determined by differential centrifugation combined with epithermal neutron activation analysis (ENAA).The analytical quality has been verified by analysis of some standard reference materials. The results show that the distribution of As in subcellular fractions of rat liver cells is not homegeneous. The highest concentration of As is found in micrososme ,while the lowest content is found in nuclei for arsenic poisoned rat .The lowest content of As is noted in mitochondria of cotrast rats .The differences of As contents in subcellular fractions of lives cells between arsenic poisoned and normal rats are compared by means of t test and exceedingly significant differeces are found ( P <0.001). In As poisoned rat, the As content in microsome is significantly higher than that in other subcellular fractions ( P <0.05),except lysosome . |
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| Keywords: | rat liver subcellular fraction epithermal neutron activation analysis arsenic |
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