Tamoxifen-induced modification of serum lipoprotein phospholipids in the cockerel |
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Authors: | W Carl Breckenridge C B Lazier |
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Affiliation: | (1) Department of Biochemistry, Dalhousie University, B3H 4H7 Halifax, Nova Scotia, Canada |
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Abstract: | The administration of tamoxifen (Tam), a nonsteroidal antiestrogen, or of a diphenyl-methane derivative of Tam that does not
bind to the estrogen receptor (DPPE) of cockerels results in a marked decrease in the concentration of serum lipoprotein constitutents
with an apparent alteration in phospholipid composition. To establish the nature of changes in phospholipids, the molecular
species of phosphatidylcholine (PC) and sphingomyelin (Sph) were isolated and characterized. Between 9 and 18 hr following
the administration of Tam or DPPE, there was a marked decrease in the proportion of molecular species of serum PC containing
C16 and C18 fatty acids, but there was an increase in the proportion of molecular species containing C20 and C22 polyunsaturated
fatty acids. Fatty acid analyses revealed that this change was due to an increase in arachidonic and docosaxaenoic acids at
the expense of oleic and linoleic acids. These proportional changes were due to an absolute decrease in serum of PC molecular
species containing palmitic and stearic acids in association with oleic and linoleic acids with very little change in the
absolute concentration of molecular species containing arachidonic and docosahexaenoic acids. By contrast, the composition
of Sph, which contained palmitic acid as the major fatty acid, was not altered during treatment. It is concluded that the
short-term effect of Tam and DPPE on plasma phospholipids of the cockerel is due to a selective conservation of PC containing
long chain polyunsaturated fatty acids. |
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