| 同位素内标-液相色谱串联质谱法测定肉松中盐酸克伦特罗 |
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| 引用本文： | 宗万里,鲁刚.同位素内标-液相色谱串联质谱法测定肉松中盐酸克伦特罗[J].生命科学仪器,2014(1):60-64. |
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| 作者姓名： | 宗万里 鲁刚 |
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| 作者单位： | 威海市产品质量监督检验所,山东威海264209 |
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| 摘 要： | 本文研究建立了肉松中盐酸克伦特罗的液相色谱串联质谱测定方法。样品中加入同位素内标稀释液后用甲醇直接提取，提取液直接注人液相色谱串联质谱仪中进行检测。利用ESI源（正离子模式），c18液相色谱柱，以及sRM方法对样品中的盐酸克伦特罗进行定性、定量分析。母离子为277，定量离子对为277〉203。该方法测定结果的相对标准偏差小于6％（n=6），平均回收率大于90％，线性范围为1．10ng／mL，最低检出限为0．0027ng／mL。
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| 关 键 词： | 液相色谱串联质谱 肉松 盐酸克伦特罗 |
Determination of Clenbuterol Hdrochloride in Dried Meat Floss by Isotope Internal Standard-LC-MS/MS |
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| Zong Wanli,Lu Gang.Determination of Clenbuterol Hdrochloride in Dried Meat Floss by Isotope Internal Standard-LC-MS/MS[J].Life Science Instruments,2014(1):60-64. |
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| Authors: | Zong Wanli Lu Gang |
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| Affiliation: | (Weihai Supervision and lnspeetion Institute of Product Quality, Weihai 264209, China) |
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| Abstract: | A method for determination of clenbuterol hdrochloride in dried meat floss by isotope internal standard-liquid chromatography-Tandem mass spectrometry（LC-MS/MS）was established. The sample added with isotope internal standard was injected into LC-MS/MS after extracted by methanol. The qualitative and quantitative analysis was achieved by ESI source under positive ion mode, C18 liquid column, SRM method. The mass of parent ion is 277, the quantitative ion pair is 277〉203 The RSD of the method was less than 6% （n=6）, and average recovery was more than 90%, the liner range was in 1-10ng/mL and the detection limit was 0.0027ng/mL |
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| Keywords: | LC-MS/MS Dried meat floss Clenbuterol |
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